ECB Room 1002 - Biomedical Experimental Teaching Lab
This lab is used for wet-lab based courses: BME 201, BME 315, BME 430, BME 511, BME 550, BME 603, and BME Design.
Please avoid using the room when a Lab is running. See calendar below. For questions related to this lab please contact John Puccinelli.
Reserve equipment:
- Request access to the google calendar (see contact)
- Title events: "Your Name-Equipment Name"
Required safety training for the lab:
For more information and other safety training courses offered at UW- see Environment, Health & Safety (EHS) - Training.
Storing items in the lab
- All project items must be stored in the back lockers and labeled with:
"Team short Name (project), Semester/Year, and Contact Information (name and email of one teammember)" - All chemicals or reagents must be labeled additionally with:
Contents and opened date
Follow this guideline for proper disposal of waste.
Room Schedule
Available Resources
General cell biology equipment
- BioSafety Cabinets (4) - Biosafety Level I
"How to guide" for Aseptic Technique - Fume Hoods (3)
- Incubators (2) at 37°C and 5% CO2 (with shaker)
"How to guide" for Incubators - Centrifuges: Eppendorf 5702/R(15 mL and 50 mL), Sorvall 17R Micro Refrigerated(1.5 mL)
- Sonicator, Ultrasonic bath, Shakers (ambient and within an incubator), Self incubating orbital shaker
- Analytical Balance, Other Balances, pH Meters
- Water baths, Oven, Hotplates/stirrers, Votexers, Micropipettors, Pipet-aids
- UVP 254 nm Oven/crosslinker
Microscopy
Nikon Ti-U Inverted Fluorescence Microscope
- "How to guide"
- Nikon Elements BR - Software manual
- Nikon - Manufacturer Webpage
- Filters: DAPI, GFP, TRITC
- Objectives: 2, 4, 10, 20, 40X
- Monochromatic camera
Schedule via scheduling the room
Olympus IX71 Inverted Fluorescence Microscope
- Filter Set Spectra
- Filters: DAPI, GFP, TexRed
- Objectives: 4, 10, 20, 40, 60X
- Monochromatic camera
Schedule via scheduling the room
Zeiss Axiovert 40 Inverted Microscope
- "How to guide"
- Zeiss - Manufacturer Webpage
- Objectives: 2, 5, 10, 20X
- Color camera (AxioCam)
Schedule via scheduling the room
Additional Microscopes
- Olympus Inverted CKX31 (4,10,20X) - no camera
- Olympus BX60 (10,20,40X) - no camera
- Nikon E400 (4,10,20,40,100X) - no camera
- Leica Stereoscope "How to guide" - eye piece camera
Mechanical Testing
MTS Criterion - Model C43
- "How to Guide"
- User Manual
- MTS - Manufacturer Webpage
- MTS Test Suite software
- Bionix EnviroBath - BioChamber and Controller (Use SS fixtures)
- Fixtures (see Insight)
Schedule via scheduling the room
MTS Insight - Model 5kN
- User Manual
- MTS - Manufacturer Webpage
- Bionix EnviroBath - BioChamber and Controller (Use SS fixtures)
- Load Cells: 50 N, 250 N, 1 kN(2), and 10 kN
- Compression fixtures: 1 kN, and 10 kN (SS)
- Tensile Grips: 100 N rubber, 100 N (SS), 2 kN, and 10 kN
- Bend Fixtures: 3 & 4 point 60 mm (SS), and 3 & 4 point 60 mm
Schedule via scheduling the room
Malvern Rheometer - Kinexus Ultra+
- User Manual
- Malvern - Manufacturer Webpage
- rSpace Software (password required)
- Geometries:
-Parallel plate: PU20 (SS)
-Cone and plate: CP 4/40 (SS)
Schedule via scheduling the room
Microfabrication
Laser Engraver/Cutter - Epilog Mini18 - 40 Watt
Schedule via scheduling the room
